Purification and Partial Characterization of Fimbriae of Vibrio Cholerae O1

Vaccine. 1987 Dec;5(4):283-8. doi: 10.1016/0264-410x(87)90153-8.

Abstract

Fimbriae of Vibrio cholerae O1 were purified from a strain of the classical biotype, Inaba serotype (Bgd 17), and a strain of the El Tor biotype, Ogawa serotype (K23), grown on TCG agar medium by the following procedure; homogenization of the cell suspension to detach fimbriae, ultracentrifugation to remove remaining cells and their debris, concentration of the supernatant containing fimbriae with ultrafiltration, and 20 to 40% sucrose linear gradient centrifugation of the concentrated material. The fimbriae in both preparations were flexible, long fibres readily distinguishable under the electron microscope from those of CFA/I, CFA/II seen in ETEC strains. Their structural subunit was a protein of 16 kdaltons. Fimbriae isolated from both serotypes and biotypes shared antigenic determinants.

MeSH terms

  • Antigens, Bacterial / analysis
  • Epitopes / analysis
  • Fimbriae, Bacterial / ultrastructure*
  • Hemagglutination
  • Hot Temperature
  • Humans
  • Microscopy, Electron
  • Neuraminidase / metabolism
  • Vibrio cholerae / isolation & purification*
  • Vibrio cholerae / ultrastructure

Substances

  • Antigens, Bacterial
  • Epitopes
  • Neuraminidase