End-binding protein 1 (EB1) up-regulation is an early event in colorectal carcinogenesis

FEBS Lett. 2014 Mar 3;588(5):829-35. doi: 10.1016/j.febslet.2014.01.046. Epub 2014 Feb 1.

Abstract

End-binding protein (EB1) is a microtubule protein that binds to the tumor suppressor adenomatous polyposis coli (APC). While EB1 is implicated as a potential oncogene, its role in cancer progression is unknown. Therefore, we analyzed EB1/APC expression at the earliest stages of colorectal carcinogenesis and in the uninvolved mucosa ("field effect") of human and animal tissue. We also performed siRNA-knockdown in colon cancer cell lines. EB1 is up-regulated in early and field carcinogenesis in the colon, and the cellular/nano-architectural effect of EB1 knockdown depended on the genetic context. Thus, dysregulation of EB1 is an important early event in colon carcinogenesis.

Keywords: Adenomatous polyposis coli; Colon cancer; End-binding protein 1; Field carcinogenesis; Sub-diffractional structure.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adenocarcinoma / genetics
  • Adenocarcinoma / metabolism*
  • Adenocarcinoma / pathology
  • Adenoma / genetics
  • Adenoma / metabolism
  • Adenoma / pathology
  • Animals
  • Apoptosis
  • Carcinogenesis / metabolism*
  • Cell Proliferation
  • Colorectal Neoplasms / genetics
  • Colorectal Neoplasms / metabolism*
  • Colorectal Neoplasms / pathology
  • Cytoskeleton / metabolism
  • Gene Expression Regulation, Neoplastic
  • HCT116 Cells
  • HT29 Cells
  • Humans
  • Male
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / metabolism*
  • Rats
  • Tissue Array Analysis
  • Up-Regulation*

Substances

  • MAPRE1 protein, human
  • Microtubule-Associated Proteins