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, 41 (4), 225-33

Incidence, Molecular Characteristics and Pathogenicity of Gibberella Fujikuroi Species Complex Associated With Rice Seeds From Asian Countries

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Incidence, Molecular Characteristics and Pathogenicity of Gibberella Fujikuroi Species Complex Associated With Rice Seeds From Asian Countries

Young-Ah Jeon et al. Mycobiology.

Abstract

Gibberella fujikuroi species complex (GFSC) was isolated from rice (Oryza sativa L.) seed samples from ten Asian countries and investigated for incidence of GFSC, molecular characteristics, and pathogenicity. Regardless of geographic origin, GFSC was detected with incidences ranging from 3% to 80%. Four species, Fusarium fujikuroi, F. concentricum, F. proliferatum, and F. verticillioides, were found to show an association with rice seeds, with F. fujikuroi being the predominant species. In phylogenetic analyses of DNA sequences, no relationship was found between species, isolates, and geographic sources of samples. Unidentified fragments of the β-tubulin gene were observed in ten isolates of F. fujikuroi and F. verticillioides. With the exception of three isolates of F. fujikuroi, F. fujikuroi, F. proliferatum, and F. verticillioides were found to have FUM1 (the fumonisin biosynthetic gene); however, FUM1 was not found in isolates of F. concentricum. Results of pathogenicity testing showed that all isolates caused reduced germination of rice seed. In addition, F. fujikuroi and F. concentricum caused typical symptoms of bakanae, leaf elongation and chlorosis, whereas F. proliferatum and F. verticillioides only caused stunting of seedlings. These findings provide insight into the characteristics of GFSC associated with rice seeds and might be helpful in development of strategies for management of bakanae.

Keywords: Gibberella fujikuroi species complex; Incidence; Molecular characteristics; Pathogenicity; Rice seed.

Figures

Fig. 1
Fig. 1
Neighbor-Joining tree based on combined DNA sequences of translation elongation factor-1α, histone H3, and β-tubulin of Gibberella fujikuroi species complex isolated from rice seed samples. The bootstrap values based on 1,000 replications are indicated as percentages in the internodes. Thick branches are supported by bootstrap values > 70%. The tree is rooted with Fusarium graminearum (NRRL 34079).
Fig. 2
Fig. 2
Neighbor-Joining tree based on DNA sequences of β-tubulin in ten isolates of Fusarium fujikuroi and F. verticillioides. β-Tubulin sequences with sizes of 360 bp and 291 bp from the same isolates were compared. The bootstrap values based on 1,000 replications are indicated as percentages in the internodes. Thick branches are supported by bootstrap values > 70%. YN09-64 had the β-tubulin sequence with maximum homology to the 360-bp fragment.
Fig. 3
Fig. 3
Pathogenicity of Gibberella fujikuroi species complex isolates to rice. Leaf elongation, chlorosis, and wide angle between leaf and stem in rice seedlings inoculated with isolates of Fusarium fujikuroi or F. concentricum (A), stunted seedlings inoculated with isolates of F. proliferatum or F. verticillioides (B), pathogenicity of each species to rice (C).

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References

    1. Ou SH. Bakanae disease and foot rot. In: Ou SH, editor. Rice diseases. Surrey, Kew: Commonwealth Mycological Institute; 1985. pp. 262–272.
    1. Sawada K. Beitrage über Formosas-Pilze no. 14. Trans Nat Hist Soc Formosa. 1917;31:31–133.
    1. Ito S, Kimura J. Studies on the 'bakanae' disease of the rice plant. Rep Hokkaido Natl Agric Exp Stn. 1931;27:1–95.
    1. Leslie JF, Summerell BA. The fusarium laboratory manual. Oxford: Blackwell Publishing; 2006.
    1. Nirenberg HI, O'Donnell K. New Fusarium species and combinations within Gibberella fujikuroi species complex. Mycologia. 1998;90:434–458.

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