Effect of Abelson murine leukemia virus on granulocytic differentiation and interleukin-3 dependence of a murine progenitor cell line

Oncogene. 1987 Mar;1(1):29-35.

Abstract

The murine diploid hematopoietic cell line 32D Cl3 strictly requires interleukin-3 (IL-3) for proliferation. When 32D Cl3 cells are transferred to IL-3-free medium which contains recombinant human granulocyte colony stimulating factor (rhG-CSF), the cell number increases four- to five-fold, and after 14 days the whole cell population is differentiated into morphologically normal and myeloperoxidase- and lactoferrin-positive metamyelocytes and granulocytes. Infection with Abelson murine leukemia virus (A-MuLV) of 32D Cl3 cells growing in the presence of IL-3 induces, within 2 weeks, the appearance of cells that are IL-3-independent for growth. The latter cells lack myeloid, T and B cell markers, and are unable to differentiate, even in the presence of very high doses of rhG-CSF. However, once the 32D Cl3 cells have been exposed to G-CSF, they become resistant to the transforming effects of A-MuLV as judged by the appearance of the IL-3-independent clones. These findings suggest that the ability of Abelson virus to transform immature progenitor cells is due to interference of the v-abl gene product with the mechanisms that control the commitment of the cells to differentiate.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Abelson murine leukemia virus / genetics*
  • Animals
  • Cell Differentiation / drug effects
  • Cell Division / drug effects
  • Cell Line
  • Cell Transformation, Viral
  • Colony-Stimulating Factors / pharmacology
  • Granulocyte Colony-Stimulating Factor
  • Granulocytes / cytology*
  • Interleukin-3 / physiology*
  • Leukemia Virus, Murine / genetics*
  • Mice
  • Oncogenes*

Substances

  • Colony-Stimulating Factors
  • Interleukin-3
  • Granulocyte Colony-Stimulating Factor