PROSAD: a powerful platform for instrument calibration and quantification

Rapid Commun Mass Spectrom. 2014 Mar 15;28(5):536-44. doi: 10.1002/rcm.6808.


Rationale: There is critical need for instrument calibration and correction procedures that can improve the quality of mass spectral quantitative results. Currently, mass spectrometry (MS) technologies suffer from certain biases related to instrumental responses, which tend to restrict its application. To overcome these biases, we developed the PROgressive SAmple Dosage (PROSAD) platform and tested it.

Methods: PROSAD, an optimized sample preparation and data analysis method, is used in conjunction with a liquid chromatography (LC)/MS system and a low-voltage ionization source (e.g., no-discharge atmospheric pressure chemical ionization (ND-APCI)). The mass spectrometers used for this report were an HCT Ultra ion trap, a LTQ XL Orbitrap, and a TSQ Vantage triple-stage quadrupole. The PROSAD elaborative system, because of its dedicated mathematical algorithm, provided a dynamic linear calibration check and correction.

Results: We tested PROSAD using a leucomalachite green-fish homogenate assay. Atrazine in tea matrix samples were also quantified. Better quantification was achieved using PROSAD compared with the classic linear, static calibration procedure in both test cases.

Conclusions: PROSAD provides a dynamically optimized calibration curve that affords increased stability, accuracy, and precision for the quantification of MS data.

MeSH terms

  • Animals
  • Atrazine / analysis
  • Calibration
  • Chromatography, Liquid / instrumentation*
  • Chromatography, Liquid / methods*
  • Fishes
  • Mass Spectrometry / instrumentation*
  • Mass Spectrometry / methods*
  • Models, Chemical
  • Reproducibility of Results
  • Rosaniline Dyes / analysis
  • Tea / chemistry


  • Rosaniline Dyes
  • Tea
  • leucomalachite green
  • Atrazine