Jak3 enables chemokine-dependent actin cytoskeleton reorganization by regulating cofilin and Rac/Rhoa GTPases activation

PLoS One. 2014 Feb 3;9(2):e88014. doi: 10.1371/journal.pone.0088014. eCollection 2014.

Abstract

We have previously shown that Jak3 is involved in the signaling pathways of CCR7, CCR9 and CXCR4 in murine T lymphocytes and that Jak3⁻/⁻ lymphocytes display an intrinsic defect in homing to peripheral lymph nodes. However, the molecular mechanism underlying the defective migration observed in Jak3⁻/⁻ lymphocytes remains elusive. Here, it is demonstrated for the first time, that Jak3 is required for the actin cytoskeleton reorganization in T lymphocytes responding to chemokines. It was found that Jak3 regulates actin polymerization by controlling cofilin inactivation in response to CCL21 and CXCL12. Interestingly, cofilin inactivation was not precluded in PTX- treated cells despite their impaired actin polymerization. Additionally, Jak3 was required for small GTPases Rac1 and RhoA activation, which are indispensable for acquisition of the migratory cell phenotype and the generation of a functional leading edge and uropod, respectively. This defect correlates with data obtained by time-lapse video-microscopy showing an incompetent uropod formation and impaired motility in Jak3-pharmacologically inhibited T lymphocytes. Our data support a new model in which Jak3 and heterotrimeric G proteins can use independent, but complementary, signaling pathways to regulate actin cytoskeleton dynamics during cell migration in response to chemokines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actin Cytoskeleton / drug effects
  • Actin Cytoskeleton / immunology
  • Actin Cytoskeleton / metabolism*
  • Actin Depolymerizing Factors / metabolism*
  • Animals
  • Cell Movement / drug effects
  • Chemokine CCL21 / pharmacology*
  • Chemokine CXCL12 / pharmacology*
  • Chemotaxis, Leukocyte / drug effects
  • Female
  • Fluorescent Antibody Technique
  • Humans
  • Janus Kinase 3 / physiology*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Microscopy, Confocal
  • Pertussis Toxin / pharmacology
  • Signal Transduction / drug effects
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / immunology
  • T-Lymphocytes / metabolism
  • Time-Lapse Imaging
  • rac GTP-Binding Proteins / metabolism*
  • rhoA GTP-Binding Protein / metabolism*

Substances

  • Actin Depolymerizing Factors
  • Chemokine CCL21
  • Chemokine CXCL12
  • Pertussis Toxin
  • Jak3 protein, mouse
  • Janus Kinase 3
  • rac GTP-Binding Proteins
  • rhoA GTP-Binding Protein

Grant support

Work in GS laboratory was supported by Grant # 79573 from Conacyt (Mexico) and DGAPA (UNAM, Mexico) Grant # 214411. Work in IM laboratory was supported by Conacyt grant #166462. Xochilt Ambriz-Peña was a student of the PhD program: Programa de Doctorado en Ciencias Biomédicas, Universidad Nacional Autónoma de México and was a recipient of a doctoral fellowship from CONACyT 23543/220705. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.