Imaging collagen degradation in vivo highlights a key role for M2-polarized macrophages in extracellular matrix degradation

Daniel H Madsen; Thomas H Bugge

doi:10.4161/onci.27127

Imaging collagen degradation in vivo highlights a key role for M2-polarized macrophages in extracellular matrix degradation

Oncoimmunology. 2013 Dec 1;2(12):e27127. doi: 10.4161/onci.27127. Epub 2013 Dec 9.

Authors

Daniel H Madsen¹, Thomas H Bugge¹

Affiliation

¹ Proteases and Tissue Remodeling Section; Oral and Pharyngeal Cancer Branch; National Institute of Dental and Craniofacial Research; National Institutes of Health; Bethesda, MD USA.

Abstract

We have recently described an assay for imaging interstitial collagen degradation in vivo, which allows for the identification of cell types and molecules involved in collagen turnover in the course of pathological and physiological tissue remodeling. The assay revealed a dominant role of receptor-mediated intracellular collagen degradation by M2-polarized macrophages in extracellular matrix turnover.

Keywords: Endo180; M2-polarized macrophages; Mrc1; Mrc2; cathepsin; collagen endocytosis; collagenase; in vivo imaging; interstitial collagen degradation; lysosome; mannose receptor; matrix metalloproteinase; tumor invasion; urokinase plasminogen activator receptor-associated protein.