Endocrine regulation of carbonate precipitate formation in marine fish intestine by stanniocalcin and PTHrP

J Exp Biol. 2014 May 1;217(Pt 9):1555-62. doi: 10.1242/jeb.098517. Epub 2014 Feb 5.

Abstract

In marine fish, high epithelial bicarbonate secretion by the intestine generates luminal carbonate precipitates of divalent cations that play a key role in water and ion homeostasis. In vitro studies highlight the involvement of the calciotropic hormones PTHrP (parathyroid hormone-related protein) and stanniocalcin (STC) in the regulation of epithelial bicarbonate transport. The present study tested the hypothesis that calciotropic hormones have a regulatory role in carbonate precipitate formation in vivo. Sea bream (Sparus aurata) juveniles received single intraperitoneal injections of piscine PTHrP(1-34), the PTH/PTHrP receptor antagonist PTHrP(7-34) or purified sea bream STC, or were passively immunized with polyclonal rabbit antisera raised against sea bream STC (STC-Ab). Endocrine effects on the expression of the basolateral sodium bicarbonate co-transporter (Slc4a4.A), the apical anion exchangers Slc26a6.A and Slc26a3.B, and the V-type proton pump β-subunit (Atp6v1b) in the anterior intestine were evaluated. In keeping with their calciotropic nature, the hypocalcaemic factors PTHrP(7-34) and STC up-regulated gene expression of all transporters. In contrast, the hypercalcaemic factor PTHrP(1-34) and STC antibodies down-regulated transporters involved in the bicarbonate secretion cascade. Changes in intestine luminal precipitate contents provoked by calcaemic endocrine factors validated these results: 24 h post-injection either PTHrP(1-34) or immunization with STC-Ab reduced the carbonate precipitate content in the sea bream intestine. In contrast, the PTH/PTHrP receptor antagonist PTHrP(7-34) increased not only the precipitated fraction but also the concentration of HCO3(-) equivalents in the intestinal fluid. These results confirm the hypothesis that calciotropic hormones have a regulatory role in carbonate precipitate formation in vivo in the intestine of marine fish. Furthermore, they illustrate for the first time in fish the counteracting effect of PTHrP and STC, and reveal an unexpected contribution of calcaemic factors to acid-base balance.

Keywords: Bicarbonate secretion; Calciotropic hormones; Calcium regulation; Endocrine regulation; Intestinal physiology; Ion transporter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acid-Base Equilibrium
  • Animals
  • Carbonates / metabolism
  • Chemical Precipitation
  • Gene Expression
  • Glycoproteins / metabolism*
  • Glycoproteins / physiology
  • Intestinal Mucosa / metabolism*
  • Ion Transport
  • Parathyroid Hormone-Related Protein / metabolism*
  • Parathyroid Hormone-Related Protein / physiology
  • Peptide Fragments / metabolism*
  • Peptide Fragments / physiology
  • Sea Bream / metabolism*

Substances

  • Carbonates
  • Glycoproteins
  • Parathyroid Hormone-Related Protein
  • Peptide Fragments
  • parathyroid hormone-related protein (1-34)
  • teleocalcin