A rolling circle amplification-based DNA machine for miRNA screening coupling catalytic hairpin assembly with DNAzyme formation

Junyang Zhuang; Wenqiang Lai; Guonan Chen; Dianping Tang

doi:10.1039/c3cc49873e

A rolling circle amplification-based DNA machine for miRNA screening coupling catalytic hairpin assembly with DNAzyme formation

Chem Commun (Camb). 2014 Mar 18;50(22):2935-8. doi: 10.1039/c3cc49873e. Epub 2014 Feb 6.

Authors

Junyang Zhuang¹, Wenqiang Lai, Guonan Chen, Dianping Tang

Affiliation

¹ Key Laboratory of Analysis and Detection of Food Safety (Ministry of Education & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350108, P.R. China. dianping.tang@fzu.edu.cn.

PMID: 24501741
DOI: 10.1039/c3cc49873e

Abstract

A novel DNA nanomachine based on the linear rolling circle amplification strategy was designed for sensitive screening of microRNA (miRNA) at an ultralow concentration coupling catalytic hairpin assembly (CHA) with DNAzyme formation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Catalysis
DNA, Catalytic*
MicroRNAs / analysis*
Nucleic Acid Amplification Techniques*

Substances

DNA, Catalytic
MicroRNAs