A Novel Viral Oncogene With Structural Similarity to Phospholipase C

Nature. 1988 Mar 17;332(6161):272-5. doi: 10.1038/332272a0.

Abstract

Numerous oncogenes have been isolated from acutely transforming retroviruses. To date, the products of these viral oncogenes have been protein kinases, nuclear proteins, growth factors, or GTP-binding proteins. We have cloned the previously uncharacterized avian sarcoma virus CT10 and sequenced its genome. This virus encodes a protein, p47gag-crk, that has blocks of sequence similarity to the amino-terminal, non-catalytic region of the non-receptor class of tyrosine kinases. In addition, the structure of p47gag-crk has striking similarity to a 180-amino acid region of bovine brain phospholipase C. Biochemical data suggest that p47gag-crk activates one or several endogenous tyrosine kinases.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Avian Sarcoma Viruses / genetics*
  • Base Sequence
  • Brain / enzymology
  • Cattle
  • Cloning, Molecular
  • DNA, Recombinant
  • DNA, Viral / genetics
  • Genes, Viral*
  • Immunosorbent Techniques
  • Molecular Sequence Data
  • Oncogenes*
  • Phosphatidylinositol Diacylglycerol-Lyase
  • Phosphoric Diester Hydrolases / genetics
  • Phosphotyrosine
  • Protein-Tyrosine Kinases / genetics
  • Sequence Homology, Nucleic Acid
  • Type C Phospholipases / genetics*
  • Tyrosine / analogs & derivatives
  • Tyrosine / metabolism

Substances

  • DNA, Recombinant
  • DNA, Viral
  • Phosphotyrosine
  • Tyrosine
  • Protein-Tyrosine Kinases
  • Phosphoric Diester Hydrolases
  • Type C Phospholipases
  • Phosphatidylinositol Diacylglycerol-Lyase

Associated data

  • GENBANK/Y00302