The genome, proteome and phylogenetic analysis of Sinorhizobium meliloti phage ΦM12, the founder of a new group of T4-superfamily phages

Virology. 2014 Feb:450-451:84-97. doi: 10.1016/j.virol.2013.11.027. Epub 2013 Dec 25.

Abstract

Phage ΦM12 is an important transducing phage of the nitrogen-fixing rhizobial bacterium Sinorhizobium meliloti. Here we report the genome, phylogenetic analysis, and proteome of ΦM12, the first report of the genome and proteome of a rhizobium-infecting T4-superfamily phage. The structural genes of ΦM12 are most similar to T4-superfamily phages of cyanobacteria. ΦM12 is the first reported T4-superfamily phage to lack genes encoding class I ribonucleotide reductase (RNR) and exonuclease dexA, and to possess a class II coenzyme B12-dependent RNR. ΦM12's novel collection of genes establishes it as the founder of a new group of T4-superfamily phages, fusing features of cyanophages and phages of enteric bacteria.

Keywords: Alphaproteobacteria; Bacteriophage; Class II ribonucleotide reductase; PhiM12; Proteome; Sinorhizobium meliloti; T4-like phage; gp20; gp23; ΦM12.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacteriophage T4 / classification*
  • Bacteriophage T4 / genetics
  • Bacteriophage T4 / isolation & purification*
  • Genome, Viral*
  • Molecular Sequence Data
  • Phylogeny*
  • Proteome / genetics*
  • Proteome / metabolism
  • Sinorhizobium meliloti / virology*
  • Viral Proteins / genetics*
  • Viral Proteins / metabolism

Substances

  • Proteome
  • Viral Proteins