Striatal adenosine levels measured 'in vivo' by microdialysis in rats with unilateral dopamine denervation

Neurosci Lett. 1987 Dec 29;83(3):338-44. doi: 10.1016/0304-3940(87)90111-x.


Adenosine, inosine, hypoxanthine and guanosine were measured in perfusates collected from the right and left striatum of halothane-anaesthetized naive and 6-hydroxydopamine-denervated rats by using microdialysis. Samples were taken under basal and KCl-stimulated conditions. Dopamine, dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) were simultaneously measured. Purines and monoamines were assayed by HPLC-UV and HPLC-EC respectively. In naive rats, basal adenosine (1 microM), inosine (2 microM), hypoxanthine (4 microM), guanosine (0.5 microM) and dopamine (DA, 0.02 microM) levels (corrected by using the in vitro % recovery of each probe) were increased by the inclusion of 100 mM of KCl into the perfusion medium (2.5-, 3-, 3.5-, 1.5- and 30-fold, respectively, while DOPAC (6 microM), HVA (5 microM) and 5-HIAA (3 microM) levels were (72%, 68% and 45% respectively). DA was strongly diminished in the denervated striatum, but when detectable, could be increased (1- to 12-fold) by KCl stimulation. Adenosine, inosine, hypoxanthine and guanosine were, however, largely unaffected by the DA denervation and could be enhanced by KCl stimulation (2-, 3-, 4- and 1.5-fold respectively). The present results indicate that: (1) as in the case for DA, there is a pool of striatal adenosine which is releasable by high concentrations of extracellular K+; however, (2) this pool of adenosine seems not to be significantly modified by mesencephalic dopamine deafferentation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3,4-Dihydroxyphenylacetic Acid / analysis
  • Adenosine / analysis*
  • Animals
  • Chromatography, High Pressure Liquid
  • Corpus Striatum / analysis*
  • Corpus Striatum / drug effects
  • Denervation
  • Dialysis / methods
  • Dopamine / analysis
  • Guanosine / analysis
  • Homovanillic Acid / analysis
  • Hydroxydopamines / pharmacology
  • Hydroxyindoleacetic Acid / analysis
  • Hypoxanthine
  • Hypoxanthines / analysis
  • Inosine / analysis
  • Male
  • Oxidopamine
  • Rats
  • Rats, Inbred Strains


  • Hydroxydopamines
  • Hypoxanthines
  • 3,4-Dihydroxyphenylacetic Acid
  • Guanosine
  • Hypoxanthine
  • Hydroxyindoleacetic Acid
  • Inosine
  • Oxidopamine
  • Adenosine
  • Dopamine
  • Homovanillic Acid