Bleomycin resistance conferred by a drug-binding protein

FEBS Lett. 1988 Mar 28;230(1-2):171-5. doi: 10.1016/0014-5793(88)80665-3.


The protein coded by a bleomycin-resistance gene (ble) cloned from producing actinomycetes was purified from a culture of a recombinant E. coli strain and its action on bleomycin was determined by in vitro assays. The protein binds reversibly in a one to one ratio to bleomycin which can no longer cleave DNA. The bleomycin resistance of cells harboring a ble gene could be accounted for by a sequestering effect of the bleomycin-binding protein.

MeSH terms

  • Actinomycetales / genetics
  • Bleomycin / metabolism*
  • Bleomycin / pharmacology
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • Carrier Proteins / pharmacology*
  • DNA / metabolism*
  • DNA, Recombinant
  • Drug Resistance, Microbial / genetics
  • Escherichia coli / genetics
  • Fluorescence
  • Osmolar Concentration


  • Carrier Proteins
  • DNA, Recombinant
  • Bleomycin
  • DNA