miR-30a suppresses breast cancer cell proliferation and migration by targeting Eya2

Biochem Biophys Res Commun. 2014 Mar 7;445(2):314-9. doi: 10.1016/j.bbrc.2014.01.174. Epub 2014 Feb 4.


Eye absent (Eya) proteins are involved in cell fate determination in a broad spectrum of cells and tissues. Aberrant expression of Eya2 has been documented in a variety of cancers and correlates with clinical outcome. However, whether microRNAs (miRNAs) can regulate Eya2 expression remains unknown. Here, we show that miR-30a represses Eya2 expression by binding to the 3'-untranslated region of Eya2. Overexpression of Eya2 in miR-30a-transfected breast cancer cells effectively rescued the inhibition of cell proliferation and migration caused by miR-30a. Knockdown of Eya2 by small-interfering RNA (siRNA) in breast cancer cells mimicked the effect induced by miR-30a and abolished the ability of miR-30a to regulate breast cancer cell proliferation and migration. The miR-30a/Eya2 axis could regulate G1/S cell cycle progression, accompanied by the modulation of expression of cell cycle-related proteins, including cyclin A, cyclin D1, cyclin E, and c-Myc. Moreover, miR-30a expression was downregulated in breast cancer patients, and negatively correlated with Eya2, which was upregulated in breast cancer patients. These data suggest that the miR-30a/Eya2 axis may play an important role in breast cancer development and progression and that miR-30a activation or Eya2 inhibition may be a useful strategy for cancer treatment.

Keywords: Breast cancer; Cell migration; Cell proliferation; Eya2; miR-30a.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Breast / metabolism
  • Breast / pathology*
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology*
  • Cell Movement
  • Cell Proliferation
  • Down-Regulation
  • Female
  • G1 Phase Cell Cycle Checkpoints
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Intracellular Signaling Peptides and Proteins / genetics*
  • Intracellular Signaling Peptides and Proteins / metabolism
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism
  • Protein Tyrosine Phosphatases / genetics*
  • Protein Tyrosine Phosphatases / metabolism


  • 3' Untranslated Regions
  • Intracellular Signaling Peptides and Proteins
  • MIRN130 microRNA, human
  • MicroRNAs
  • Nuclear Proteins
  • EYA2 protein, human
  • Protein Tyrosine Phosphatases