Visualizing genomes with Oligopaint FISH probes

Curr Protoc Mol Biol. 2014 Jan 6;105:Unit 14.23.. doi: 10.1002/0471142727.mb1423s105.

Abstract

Oligopaint probes are fluorescently labeled, single-stranded DNA oligonucleotides that can be used to visualize genomic regions ranging in size from tens of kilobases to many megabases. This unit details how Oligopaint probes can be synthesized using basic molecular biological techniques, and provides protocols for FISH, 3D-FISH, and sample preparation.

Keywords: FISH; Oligopaint; complex DNA library; oligonucleotide.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Single-Stranded / genetics*
  • Genome / genetics*
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Oligonucleotide Probes / genetics*
  • Oligonucleotides / genetics*

Substances

  • DNA, Single-Stranded
  • Oligonucleotide Probes
  • Oligonucleotides