A dark brown roast coffee blend is less effective at stimulating gastric acid secretion in healthy volunteers compared to a medium roast market blend

Mol Nutr Food Res. 2014 Jun;58(6):1370-3. doi: 10.1002/mnfr.201300890. Epub 2014 Feb 8.


Coffee consumption sometimes is associated with symptoms of stomach discomfort. This work aimed to elucidate whether two coffee beverages, containing similar amounts of caffeine, but differing in their concentrations of (β) N-alkanoyl-5-hydroxytryptamides (C5HTs), chlorogenic acids (CGAs), trigonelline, and N-methylpyridinium (N-MP) have different effects on gastric acid secretion in healthy volunteers. The intragastric pH after administration of bicarbonate with/without 200 mL of a coffee beverage prepared from a market blend or dark roast blend was analyzed in nine healthy volunteers. Coffee beverages were analyzed for their contents of C5HT, N-MP, trigonelline, CGAs, and caffeine using HPLC-DAD and HPLC-MS/MS. Chemical analysis revealed higher concentrations of N-MP for the dark brown blend (87 mg/L) compared to the market blend coffee (29 mg/L), whereas concentrations of C5HT (0.012 versus 0.343 mg/L), CGAs (323 versus 1126 mg/L), and trigonelline (119 versus 343 mg/L) were lower, and caffeine concentrations were similar (607 versus 674 mg/mL). Gastric acid secretion was less effectively stimulated after administration of the dark roast blend coffee compared to the market blend. Future studies are warranted to verify whether a high ratio of N-MP to C5HT and CGAs is beneficial for reducing coffee-associated gastric acid secretion.

Keywords: Chlorogenic acids; Coffee; N-methylpyridinium; Telemetric gastric pH measurement; βN-alkanoyl-5-hydroxytryptamides.

Publication types

  • Comparative Study
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Alkaloids / administration & dosage
  • Alkaloids / analysis
  • Caffeine / administration & dosage
  • Caffeine / analysis
  • Chlorogenic Acid / administration & dosage
  • Chlorogenic Acid / analysis
  • Chromatography, High Pressure Liquid
  • Coffee / chemistry*
  • Double-Blind Method
  • Female
  • Gastric Acid / metabolism*
  • Healthy Volunteers
  • Humans
  • Hydrogen-Ion Concentration
  • Male
  • Niacinamide / administration & dosage
  • Niacinamide / analogs & derivatives
  • Niacinamide / analysis
  • Pyridinium Compounds / administration & dosage
  • Pyridinium Compounds / analysis
  • Tandem Mass Spectrometry
  • Tryptamines / administration & dosage
  • Tryptamines / analysis
  • Young Adult


  • Alkaloids
  • Coffee
  • Pyridinium Compounds
  • Tryptamines
  • Niacinamide
  • Chlorogenic Acid
  • Caffeine
  • trigonelline
  • 1-methylpyridinium