Electroporation-mediated intradermal delivery of DNA vaccines in nonhuman primates

Methods Mol Biol. 2014;1121:309-13. doi: 10.1007/978-1-4614-9632-8_27.

Abstract

Strategies to improve vaccine efficacy are still required. The immunogenicity of DNA vaccines is strongly improved by electroporation (EP). The skin is populated with a wide variety of immune cells, making it an attractive tissue for vaccine delivery. Here we describe a method for the EP-mediated intradermal delivery of DNA vaccines in nonhuman primates, as a model for preclinical development of human vaccines, using noninvasive needleless electrodes.

MeSH terms

  • Animals
  • Electroporation / methods*
  • Injections, Intradermal
  • Macaca*
  • Vaccines, DNA / administration & dosage*
  • Vaccines, DNA / metabolism*

Substances

  • Vaccines, DNA