The crystal structure of novel chondroitin lyase ODV-E66, a baculovirus envelope protein

Yoshirou Kawaguchi; Nobuo Sugiura; Koji Kimata; Makoto Kimura; Yoshimitu Kakuta

doi:10.1016/j.febslet.2013.10.021

The crystal structure of novel chondroitin lyase ODV-E66, a baculovirus envelope protein

FEBS Lett. 2013 Oct 25:S0014-5793(13)00778-3. doi: 10.1016/j.febslet.2013.10.021. Online ahead of print.

Authors

Yoshirou Kawaguchi¹, Nobuo Sugiura², Koji Kimata³, Makoto Kimura⁴, Yoshimitu Kakuta⁵

Affiliations

¹ Laboratory of Structural Biology, Graduate School of System Life Sciences, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
² Institute for Molecular Science of Medicine, Aichi Medical University, 1-1 Yazakokarimata, Nagakute, Aichi 480-1195, Japan.
³ Research Complex for the Medicine Frontiers, Aichi Medical University, 1-1 Yazakokarimata, Nagakute, Aichi 480-1195, Japan.
⁴ Laboratory of Structural Biology, Graduate School of System Life Sciences, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan; Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
⁵ Laboratory of Structural Biology, Graduate School of System Life Sciences, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan; Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan. Electronic address: kakuta@agr.kyushu-u.ac.jp.

PMID: 24512853
DOI: 10.1016/j.febslet.2013.10.021

Abstract

Chondroitin lyases have been known as pathogenic bacterial enzymes that degrade chondroitin. Recently, baculovirus envelope protein ODV-E66 was identified as the first reported viral chondroitin lyase. ODV-E66 has low sequence identity with bacterial lyases at <12%, and unique characteristics reflecting the life cycle of baculovirus. To understand ODV-E66's structural basis, the crystal structure was determined and it was found that the structural fold resembled that of polysaccharide lyase 8 proteins and that the catalytic residues were also conserved. This structure enabled discussion of the unique substrate specificity and the stability of ODV-E66 as well as the host specificity of baculovirus.

Keywords: Autographa californica; Chondroitin sulfate; Interdomain movement; ODV-E66; PL8; Polysaccharide lyase; occlusion derived virus envelope protein 66; polysaccharide lyase 8; β-Elimination.