Resolution of a Gen-2 handheld optical imager: diffuse and fluorescence imaging studies

Appl Opt. 2013 Nov 20;52(33):8060-6. doi: 10.1364/AO.52.008060.


A generation-2 (Gen-2) handheld optical imager capable of two-dimensional surface and three-dimensional tomographic imaging has recently been developed. Herein, the ability of the handheld imager to detect and resolve two targets under diffuse and fluorescence imaging conditions has been demonstrated via tissue phantom studies. Two-dimensional surface imaging studies demonstrated that two 0.96 cm diameter Indocyannine Green targets were detected and resolved ~0.5 cm apart (between edges) at a target depth of 1 cm during diffuse imaging and up to 2 cm depth during fluorescence imaging. Preliminary 3D tomographic imaging capability to resolve the two targets was also demonstrated, but requires extensive future studies.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Equipment Design
  • Equipment Failure Analysis
  • Fiber Optic Technology / instrumentation*
  • Image Enhancement / instrumentation*
  • Image Interpretation, Computer-Assisted / instrumentation*
  • Microscopy, Fluorescence / instrumentation*
  • Miniaturization
  • Point-of-Care Systems*
  • Tomography, Optical / instrumentation*