First isolation and molecular characterization of Toxoplasma gondii from a human placenta in Argentina

Parasitol Int. 2014 Apr;63(2):470-2. doi: 10.1016/j.parint.2013.10.011. Epub 2013 Oct 26.

Abstract

Blood sample and placenta were taken from a 37-week pregnant woman; serologic results indicated acute toxoplasmosis. Placenta was inoculated into mice. Seropositive mice were sacrificed and tissue cysts from brain were inoculated into new mice. Specific DNA was detected by PCR, and the isolate was characterized as Type II by nPCR-RFLP for nSAG2, SAG3, BTUB, GRA6, c29-2, c22-8, L358, PK1 and Apico markers. This is the first isolation and molecular characterization of Toxoplasma gondii from humans in Argentina.

Keywords: Genotyping; Human; Isolation; Placenta; Toxoplasma gondii.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Argentina / epidemiology
  • Biological Assay
  • Coccidiostats / therapeutic use
  • Female
  • Humans
  • Infectious Disease Transmission, Vertical*
  • Mice
  • Placenta / parasitology*
  • Pregnancy
  • Spiramycin / therapeutic use
  • Toxoplasma / isolation & purification*
  • Toxoplasmosis / drug therapy
  • Toxoplasmosis / epidemiology*
  • Toxoplasmosis / transmission*

Substances

  • Coccidiostats
  • Spiramycin