Coupling mRNA processing with transcription in time and space

Nat Rev Genet. 2014 Mar;15(3):163-75. doi: 10.1038/nrg3662. Epub 2014 Feb 11.


Maturation of mRNA precursors often occurs simultaneously with their synthesis by RNA polymerase II (Pol II). The co-transcriptional nature of mRNA processing has permitted the evolution of coupling mechanisms that coordinate transcription with mRNA capping, splicing, editing and 3' end formation. Recent experiments using sophisticated new methods for analysis of nascent RNA have provided important insights into the relative amount of co-transcriptional and post-transcriptional processing, the relationship between mRNA elongation and processing, and the role of the Pol II carboxy-terminal domain (CTD) in regulating these processes.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • RNA Caps
  • RNA Polymerase II / genetics
  • RNA Polymerase II / metabolism
  • RNA Splicing
  • RNA, Messenger / genetics*
  • Transcription, Genetic*


  • RNA Caps
  • RNA, Messenger
  • RNA Polymerase II