Cloning of recombinant monoclonal antibodies from hybridomas in a single mammalian expression plasmid

Methods Mol Biol. 2014;1131:229-40. doi: 10.1007/978-1-62703-992-5_14.

Abstract

Antibodies are an integral part of biological and medical research. In addition, immunoglobulins are used in many diagnostic tests and are becoming increasingly important in the therapy of diseases. To express antibodies recombinantly, the immunoglobulin heavy and light chains are usually cloned into two different expression plasmids. Here, we describe a method for recombinant antibody expression from a single plasmid.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / genetics
  • Antibodies, Monoclonal / metabolism*
  • Humans
  • Hybridomas / metabolism
  • Immunoglobulin Heavy Chains / genetics
  • Immunoglobulin Heavy Chains / metabolism
  • Immunoglobulin Light Chains / genetics
  • Immunoglobulin Light Chains / metabolism
  • Plasmids / genetics
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism*

Substances

  • Antibodies, Monoclonal
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Light Chains
  • Recombinant Proteins