Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014;1131:315-34.
doi: 10.1007/978-1-62703-992-5_20.

Reformatting of scFv Antibodies Into the scFv-Fc Format and Their Downstream Purification

Affiliations

Reformatting of scFv Antibodies Into the scFv-Fc Format and Their Downstream Purification

Emil Bujak et al. Methods Mol Biol. .

Abstract

The scFv-Fc format allows for rapid characterization of candidate scFvs isolated from phage display libraries before conversion into a full-length IgG. This format offers several advantages over the phage display-derived scFv, including bivalent binding, longer half-life, and Fc-mediated effector functions. Here, a detailed method is presented, which describes the cloning, expression, and purification of an scFv-Fc fragment, starting from scFv fragments obtained from a phage display library. This method facilitates the rapid screening of candidate antibodies, prior to a more time-consuming conversion into a full IgG format. Alternatively, the scFv-Fc format may be used in the clinic for therapeutic applications.

Similar articles

See all similar articles

Cited by 8 articles

See all "Cited by" articles

MeSH terms

Substances

LinkOut - more resources

Feedback