The Influence of Exogenous Antigenic Stimulation on the Specificity Repertoire of Background Immunoglobulin-Secreting Cells of Different Isotypes

Cell Immunol. 1988 Apr 1;112(2):371-80. doi: 10.1016/0008-8749(88)90306-1.

Abstract

The total number of spontaneously occurring ("background") IgM-, IgG-, and IgA-secreting cells and the frequency of antigen-specific IgM-, IgG-, and IgA-secreting cells were determined in germ-free BALB/c mice fed a chemically defined ultrafiltered diet (GF-CD), in specific pathogen-free BALB/c mice fed an autoclaved natural ingredient diet (SPF-NI), and in conventional BALB/c mice fed nonautoclaved natural ingredients (CV-NI). This was done by means of the ELISA-plaque assay. The results did not show differences among the various groups of mice with regard to the total numbers of IgM-secreting cells in the various lymphoid organs. Also the frequencies of IgM-secreting cells specific for DNP27-BSA and the anti-idiotypic monoclonal antibodies Ac38 and Ac146 did not differ significantly among GF-CD, SPF-NI, and CV-NI mice. GF-CD mice, however, did show substantially decreased numbers of IgG- and IgA-secreting cells in their lymphoid organs. Furthermore, there were striking differences in the frequencies of antigen-specific IgG- and IgA-secreting cells between GF-CD mice and the two other groups of mice. These results indicate that exogenous antigenic stimulation has a great effect on both the total numbers and the specificity repertoires of background IgG- and IgA-secreting cells. Such an influence could not be detected with regard to the background IgM-secreting cells. This suggests two distinct compartments of background Ig-secreting cells: a very stable, endogenously regulated compartment consisting mainly of IgM-secreting cells, and another compartment, consisting mainly of IgG- and IgA-secreting cells, whose numbers and specificity repertoire appeared to be influenced by exogenous antigenic stimulation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibody-Producing Cells / classification*
  • Antibody-Producing Cells / immunology
  • Antibody-Producing Cells / metabolism
  • Dinitrophenols / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / immunology*
  • Female
  • Haptens / immunology
  • Hemolytic Plaque Technique
  • Immunoglobulin Idiotypes / immunology
  • Immunoglobulin Isotypes / biosynthesis*
  • Lymphoid Tissue / cytology
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Serum Albumin, Bovine / immunology
  • Staphylococcal Protein A

Substances

  • Dinitrophenols
  • Epitopes
  • Haptens
  • Immunoglobulin Idiotypes
  • Immunoglobulin Isotypes
  • Staphylococcal Protein A
  • dinitrophenyl-bovine serum albumin
  • Serum Albumin, Bovine