Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

C A G Leal; G A Carvalho-Castro; A C Cottorello; R C Leite; H C P Figueiredo

doi:10.1590/S1517-83822013005000054

Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

Braz J Microbiol. 2013 Nov 15;44(3):901-4. doi: 10.1590/S1517-83822013005000054. eCollection 2013.

Authors

C A G Leal¹, G A Carvalho-Castro¹, A C Cottorello², R C Leite¹, H C P Figueiredo¹

Affiliations

¹ Laboratório de Doenças de Animais Aquáticos, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
² Laboratório Nacional Agropecuário, Ministério da Agricultura, Pedro Leopoldo, MG, Brazil.

Abstract

The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.

Keywords: WSD; WSSV; clinical samples; real-time PCR; sensitivity.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Molecular Diagnostic Techniques / methods*
Penaeidae / virology*
Real-Time Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Veterinary Medicine / methods*