Highly stable colorimetric aptamer sensors for detection of ochratoxin A through optimizing the sequence with the covalent conjugation of hemin

Analyst. 2014 Apr 7;139(7):1622-7. doi: 10.1039/c3an01639k.

Abstract

Optimization of hairpin DNA is introduced to detect ochratoxin A (OTA) by chemically conjugating its cofactor, hemin, toward the 5'-end. The newly designed OTA aptasensor showed enhanced stability and sensitivity, thereby lowering the detection limit to an ~1 nM level. Furthermore, an optimal spacer for hemin conjugation was investigated for stable responses toward very diluted OTA solutions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Base Sequence
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • Colorimetry / instrumentation
  • Colorimetry / methods*
  • Hemin / chemistry*
  • Limit of Detection
  • Molecular Sequence Data
  • Ochratoxins / analysis*

Substances

  • Aptamers, Nucleotide
  • Ochratoxins
  • ochratoxin A
  • Hemin