Antibodies that inhibit fusion of human immunodeficiency virus-infected cells bind a 24-amino acid sequence of the viral envelope, gp120

Proc Natl Acad Sci U S A. 1988 May;85(9):3198-202. doi: 10.1073/pnas.85.9.3198.


Antisera to recombinant human immunodeficiency virus (HIV) proteins containing the entire envelope, gp160, or the central portion of the envelope, PB1, can inhibit fusion of virally infected cells in culture. This fusion inhibition is HIV-variant specific--that is, anti-gp160-IIIB inhibits fusion of isolate HTLV-IIIB-infected cells but not of isolate HTLV-IIIRF-infected cells. Both anti-gp160 and anti-PB1 are completely blocked in fusion inhibition activity by the addition of PB1 protein. A 24-amino acid peptide (RP135, amino acids 307-330) completely blocks fusion inhibition activity of both antisera and also blocks the activity of serum from a chimpanzee infected with HTLV-IIIB. Thus, the principal epitope that elicits fusion-inhibiting antibodies is located in the central portion of gp120.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blood
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / analysis
  • HIV / immunology*
  • HIV Envelope Protein gp120
  • Pan troglodytes
  • Retroviridae Proteins / immunology*
  • Viral Fusion Proteins / antagonists & inhibitors


  • Epitopes
  • HIV Envelope Protein gp120
  • Retroviridae Proteins
  • Viral Fusion Proteins