Immunosuppressive effects of fisetin against dinitrofluorobenzene-induced atopic dermatitis-like symptoms in NC/Nga mice

Food Chem Toxicol. 2014 Apr;66:341-9. doi: 10.1016/j.fct.2014.01.057. Epub 2014 Feb 10.

Abstract

Atopic dermatitis (AD) is a multifactorial chronic skin disorder that is increasing in prevalence globally. In NC/Nga mice, repetitive epicutaneous applications of 2-4-dinitrofluorobenzene (DNFB) induces AD-like clinical symptoms. Bioflanonol fisetin (3,7,3',4'-tetrahydroxyflavone) is a dietary component found in plants, fruits and vegetables. Fisetin has various physiological effects that include anti-oxidation, anti-angiogenesis, anti-carcinogenesis and anti-inflammation. In this study, we investigated whether fisetin relieves AD-like clinical symptoms induced by repeated DNFB treatment in NC/Nga mice. Fisetin significantly inhibited infiltration of inflammatory cells including eosinophils, mast cells and CD4(+) T and CD8(+) T cells, and suppressed the expressions of cytokines and chemokines associated with dermal infiltrates in AD-like skin lesions. Total serum immunoglobulin E (IgE) levels and the ratio of phospho-NF-κB p65 to total NF-κB p65 were markedly reduced by fisetin. Fisetin also reduced the production of interferon-gamma and interleukin-4 by activated CD4(+) T cells in a dose-dependent manner, whereas the anti-inflammatory cytokine, interleukin-10 was increased. These results implicate fisetin as a potential therapeutic for AD.

Keywords: 2-4-Dinitrofluorobenzene; Atopic dermatitis; Fisetin; NC/Nga; NF-κB.

MeSH terms

  • Animals
  • Base Sequence
  • CD4-Positive T-Lymphocytes / metabolism
  • DNA Primers
  • Dermatitis, Atopic / etiology*
  • Dinitrofluorobenzene / toxicity*
  • Flavonoids / pharmacology*
  • Immunoglobulin E / blood
  • Immunosuppressive Agents / pharmacology*
  • Male
  • Mice
  • Polymerase Chain Reaction

Substances

  • DNA Primers
  • Flavonoids
  • Immunosuppressive Agents
  • Immunoglobulin E
  • Dinitrofluorobenzene
  • fisetin