Exosome-formed synthetic microRNA-143 is transferred to osteosarcoma cells and inhibits their migration

Biochem Biophys Res Commun. 2014 Mar 7;445(2):381-7. doi: 10.1016/j.bbrc.2014.02.007. Epub 2014 Feb 10.


MicroRNAs (miRNAs) have emerged as potential anticancer agents, but their clinical application is limited by the lack of an effective delivery system to tumors. Exosomes are small vesicles that play important roles in intercellular communication. Here, we show that synthetic miR-143 introduced into cells is released enveloped in exosomes and that the secreted exosome-formed miR-143 is transferred to osteosarcoma cells. The delivery of exosome-formed miR-143 significantly reduced the migration of osteosarcoma cells. The delivery efficiency of exosome-formed miR-143 was less than that achieved with lipofection, but the migratory potential of osteosarcoma cells was similarly inhibited after both strategies. Our results suggest that exosomes can deliver synthetic miR-143 and are a potentially efficient and functional delivery system.

Keywords: Delivery system; Exosome; Osteosarcoma cells; miR-143.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Cell Movement*
  • Exosomes / metabolism*
  • Humans
  • Mesenchymal Stem Cells / metabolism
  • MicroRNAs / administration & dosage*
  • MicroRNAs / genetics
  • MicroRNAs / metabolism
  • MicroRNAs / therapeutic use
  • Neoplasm Metastasis / pathology
  • Neoplasm Metastasis / prevention & control*
  • Osteosarcoma / genetics
  • Osteosarcoma / pathology*
  • Osteosarcoma / therapy
  • Transfection


  • MIRN143 microRNA, human
  • MicroRNAs