Evidence for expression and function of angiotensin II receptor type 1 in pulmonary epithelial cells

Respir Physiol Neurobiol. 2014 May 1;195:37-40. doi: 10.1016/j.resp.2014.02.001. Epub 2014 Feb 12.


There is a growing evidence that the peptide hormone angiotensin II (ANGII) can act as an auto-/paracrine mediator to regulate epithelial ion transport processes. The present study focused on the impact of ANGII on transepithelial ion transport in pulmonary epithelia. Transcripts for the ANGII receptor type 1 (ATR1) were detected in lungs of Xenopus laevis and H441 cells (human pulmonary epithelial cell line). Native Xenopus lung preparations were used for Ussing chamber recordings and apically applied ANGII (10μM) induced a significant increase of short-circuit current (ISC: 8±2%, n=13). Pre-incubation with losartan (LOS), an antagonist of ATR1 prevented the effect of ANGII on ISC. Transcripts for ATR1 in Xenopus lungs and H441 cells were detected and an increase of ISC was observed by ANGII in native Xenopus lung epithelia. This indicates that ANGII is a potential auto-/paracrine mediator for ion transport regulation in pulmonary epithelia.

Keywords: Angiotensin II; Angiotensin II receptor; Pulmonary epithelium; Pulmonary ion transport; Ussing chamber; Xenopus laevis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiotensin II / metabolism
  • Angiotensin II Type 1 Receptor Blockers / pharmacology
  • Animals
  • Cell Line
  • Epithelial Cells / drug effects
  • Epithelial Cells / physiology*
  • Female
  • Humans
  • In Vitro Techniques
  • Ion Transport / drug effects
  • Ion Transport / physiology
  • Losartan / pharmacology
  • Lung / drug effects
  • Lung / physiology*
  • Receptor, Angiotensin, Type 1 / metabolism*
  • Respiratory Mucosa / drug effects
  • Respiratory Mucosa / physiology*
  • Xenopus Proteins / metabolism*
  • Xenopus laevis


  • AGTR1 protein, Xenopus
  • AGTR1 protein, human
  • Angiotensin II Type 1 Receptor Blockers
  • Receptor, Angiotensin, Type 1
  • Xenopus Proteins
  • Angiotensin II
  • Losartan