Remineralization efficiency of bioactive glass on artificially induced carious lesion an in-vitro study

J Indian Soc Pedod Prev Dent. 2014 Jan-Mar;32(1):19-25. doi: 10.4103/0970-4388.127047.


Objectives: The objective of this study is to investigate the efficacy of bioactive glass containing product on remineralization of artificial induced carious enamel lesion and to compare its efficiency with other remineralization products using an in-vitro pH cycling method. The null hypothesis tested was bioactive glass has no effect on enamel remineralization.

Materials and methods: A total of 20 enamel samples of human molar teeth were subjected to artificial caries lesion formation using pH cycling method and was verified using high resolution scanning electron microscope (HRSEM). Each demineralized sample was then divided into five test groups each containing twenty. Group A - Bioactive glass (SHY-NM), Group B - Fluoride tooth paste (Amflor), Group C - CPP-ACP (Tooth mousse), Group D - CPP-ACPF (Tooth mousse plus), Group E - control. All the test groups were exposed to the pH cycling regime, the remineralizing agents were applied for 10 min except control. After 10 days period, the entire test groups were evaluated with HRSEM and quantitative assessment by energy dispersive X-ray spectroscopy. The obtained data was analyzed statistically using one-way ANOVA, Student's t-test and Tukey's multiple comparison tests. P ≤ 0.05 was considered to be significant.

Results: Rejection of the null hypothesis and highlights the concept of biomimetic bioactive glass as an effective remineralizing agent.

Clinical relevance: To focus on the importance of minimal invasive treatment on incipient carious lesion by remineralization.

MeSH terms

  • Biocompatible Materials
  • Dental Caries / therapy*
  • Glass*
  • Humans
  • In Vitro Techniques
  • Microscopy, Electron, Scanning
  • Molar
  • Spectrometry, X-Ray Emission
  • Tooth Remineralization*


  • Biocompatible Materials