Lipidomic "deep profiling": an enhanced workflow to reveal new molecular species of signaling lipids

Anal Chem. 2014 Mar 18;86(6):3043-7. doi: 10.1021/ac4039652. Epub 2014 Feb 27.


Current mass spectrometry-based lipidomics aims to comprehensively cover wide ranges of lipid classes. We introduce a strategy to capture phospho-monoester lipids and improve the detection of long-chain base phosphates (LCB-Ps, e.g., sphingosine-1-phosphate). Ten novel LCB-Ps (d18:2, t20:1, odd carbon forms) were discovered and characterized in tissues from human and mouse, as well in D. melanogaster and S. cerevisiae. These findings have immediate relevance for our understanding of sphingosine-1-phosphate biosynthesis, signaling, and degradation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Drosophila melanogaster
  • Humans
  • Lipids / chemistry*
  • Mass Spectrometry
  • Mice
  • Saccharomyces cerevisiae
  • Signal Transduction*


  • Lipids