Regulation of recombinant Trichinella spiralis 53-kDa protein (rTsP53) on alternatively activated macrophages via STAT6 but not IL-4Rα in vitro

Cell Immunol. Mar-Apr 2014;288(1-2):1-7. doi: 10.1016/j.cellimm.2014.01.010. Epub 2014 Feb 6.

Abstract

Classically activated macrophages (M1) or alternatively activated macrophages (M2) have different functions during helminth infections including Trichinella spiralis (T. spiralis). The excretory/secretory antigens (ESA) of T. spiralis can inhibit macrophage pro-inflammatory cytokines production. However, the specific molecules of ESA that regulate macrophages have not been identified. We previously reported that recombinant T. spiralis derived molecule 53-kDa protein (rTsP53) had protected mice from colitis. Furthermore, in the present study in vitro, we investigated rTsP53 showed anti-inflammatory function by inducing peritoneal macrophages to M2 with expressing M2 molecules of mannose receptor (MR), a novel mammalian lectin (Ym1), arginase-1 (Arg1), and interleukin (IL)-10. Next, we found the effect of rTsP53 on M2 independently of IL-4Rα. But rTsP53 can act dependently on signal transducers and activators of transcription 6 (STAT6). These results further imply that rTsP53 has potential as prospective immuno-therapeutics for inflammatory disorders.

Keywords: 53-kDa protein; Excretory/secretory antigen; Macrophage phenotype; Trichinella spiralis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Helminth / genetics
  • Antigens, Helminth / immunology
  • Antigens, Helminth / pharmacology*
  • Arginase / genetics
  • Arginase / immunology
  • Female
  • Gene Expression Regulation
  • Helminth Proteins / genetics
  • Helminth Proteins / immunology
  • Helminth Proteins / pharmacology*
  • Host-Parasite Interactions
  • Interleukin-10 / genetics
  • Interleukin-10 / immunology
  • Lectins / genetics
  • Lectins / immunology
  • Lipopolysaccharides / pharmacology
  • Macrophages, Peritoneal / drug effects*
  • Macrophages, Peritoneal / immunology
  • Macrophages, Peritoneal / parasitology
  • Mice
  • Mice, Inbred BALB C
  • Primary Cell Culture
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / immunology
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Recombinant Proteins / pharmacology
  • STAT6 Transcription Factor / genetics*
  • STAT6 Transcription Factor / immunology
  • Signal Transduction
  • Trichinella spiralis / chemistry*
  • Trichinella spiralis / genetics
  • beta-N-Acetylhexosaminidases / genetics
  • beta-N-Acetylhexosaminidases / immunology

Substances

  • Antigens, Helminth
  • Helminth Proteins
  • IL10 protein, mouse
  • Il4ra protein, mouse
  • Lectins
  • Lipopolysaccharides
  • Receptors, Cell Surface
  • Recombinant Proteins
  • STAT6 Transcription Factor
  • Stat6 protein, mouse
  • Interleukin-10
  • Chil3 protein, mouse
  • beta-N-Acetylhexosaminidases
  • Arg1 protein, mouse
  • Arginase