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. 2014:21:6.
doi: 10.1051/parasite/2014007. Epub 2014 Feb 19.

Molecular characterization of Toxoplasma gondii Type II in sheep abortion in Sardinia, Italy

Giovanna Chessa  1 , Valentina Chisu  1 , Rosaura Porcu  1 , Giovanna Masala  1
Affiliations

Molecular characterization of Toxoplasma gondii Type II in sheep abortion in Sardinia, Italy

Giovanna Chessa et al. Parasite. 2014.

Abstract
in English, French

During 2009-2010, 161 tissue samples (142 placentas, 16 brains, and 3 livers) from aborted ovine fetuses on Sardinia Island, Italy, were tested for toxoplasmosis. Organs that showed a positive result by nested polymerase chain reaction (PCR) targeting the ITS1 region for Toxoplasma gondii were also amplified with 11 genetic markers (SAG1, 5'-SAG2, 3'-SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico) and then subjected to PCR/RFLP for genetic typing. T. gondii DNA was found in 5 placentas, 14 brains, and 2 livers by PCR analysis and all isolates displayed Type II alleles at all 11 loci with all 11 markers. The results indicate that the Type II T. gondii is associated with ovine abortion.

Pendant la période 2009–2010, 161 échantillons de tissus (142 placentas, 16 cervelles et 3 foies) provenant de fœtus ovins avortés de Sardaigne, Italie, ont été testés pour la toxoplasmose. Les organes trouvés positifs par PCR visant la région ITS1 de Toxoplasma gondii ont été amplifiés avec 11 marqueurs génétiques (SAG1, 5′-SAG2, 3′-SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 et Apico) et ensuite soumis à PCR/RFLP pour génotypage. De l’ADN de T. gondii a été trouvé dans 5 placentas, 14 cervelles et 2 foies par les analyses PCR et tous les isolats avaient des allèles de Type II pour les 11 loci avec tous les 11 marqueurs. Les résultats indiquent que T. gondii de Type II est associé avec les avortements ovins.

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