Regulation of the number of alpha-bungarotoxin binding sites in cultured chick myotubes by a 1,4 dihydropyridine calcium channel antagonist

J Neurosci Res. 1988 Mar;19(3):321-5. doi: 10.1002/jnr.490190307.

Abstract

Calcium has been suggested as the second messenger link between skeletal muscle activity and AChR gene expression and synthesis. We have compared the concentrations of the Ca2+ channel antagonists D600 and nisoldipine needed both to block Ca2+ uptake into cultured myotubes and to increase AChR expression. The good correspondence between these two measurements and the use of the highly specific Ca2+ channel antagonist nisoldipine strengthens the hypothesis that AChR expression is regulated by levels of intracellular Ca2+.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / pharmacology
  • Animals
  • Bungarotoxins / metabolism*
  • Calcium / metabolism
  • Calcium Channel Blockers / pharmacology*
  • Cells, Cultured
  • Chick Embryo
  • Gallopamil / pharmacology*
  • Kinetics
  • Muscles / metabolism*
  • Nifedipine / analogs & derivatives
  • Nifedipine / pharmacology
  • Nisoldipine
  • Receptors, Cholinergic / drug effects
  • Receptors, Cholinergic / metabolism*

Substances

  • Bungarotoxins
  • Calcium Channel Blockers
  • Receptors, Cholinergic
  • Gallopamil
  • Nisoldipine
  • 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester
  • Nifedipine
  • Calcium