Characterization of human papillomavirus type 154 and tissue tropism of gammapapillomaviruses

PLoS One. 2014 Feb 13;9(2):e89342. doi: 10.1371/journal.pone.0089342. eCollection 2014.

Abstract

The novel human papillomavirus type 154 (HPV154) was characterized from a wart on the crena ani of a three-year-old boy. It was previously designated as the putative HPV type FADI3 by sequencing of a subgenomic FAP amplicon. We obtained the complete genome by combined methods including rolling circle amplification (RCA), genome walking through an adapted method for detection of integrated papillomavirus sequences by ligation-mediated PCR (DIPS-PCR), long-range PCR, and finally by cloning of four overlapping amplicons. Phylogenetically, the HPV154 genome clustered together with members of the proposed species Gammapapillomavirus 11, and demonstrated the highest identity in L1 to HPV136 (68.6%). The HPV154 was detected in 3% (2/62) of forehead skin swabs from healthy children. In addition, the different detection sites of 62 gammapapillomaviruses were summarized in order to analyze their tissue tropism. Several of these HPV types have been detected from multiple sources such as skin, oral, nasal, and genital sites, suggesting that the gammapapillomaviruses are generalists with a broader tissue tropism than previously appreciated. The study expands current knowledge concerning genetic diversity and tropism among HPV types in the rapidly growing gammapapillomavirus genus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Buttocks / virology
  • Child, Preschool
  • DNA, Viral / classification
  • DNA, Viral / genetics*
  • Female
  • Forehead / virology
  • Gammapapillomavirus / classification
  • Gammapapillomavirus / genetics*
  • Gammapapillomavirus / isolation & purification
  • Genetic Variation
  • Genome, Viral*
  • Humans
  • Infant
  • Male
  • Molecular Sequence Data
  • Papillomavirus Infections / virology*
  • Phylogeny*
  • Viral Tropism
  • Warts*

Substances

  • DNA, Viral

Associated data

  • GENBANK/AB331650
  • GENBANK/AB543507
  • GENBANK/AB646346
  • GENBANK/AF486184
  • GENBANK/AJ238373
  • GENBANK/AJ620208
  • GENBANK/AJ620210
  • GENBANK/AY722648
  • GENBANK/AY956402
  • GENBANK/DQ080078
  • GENBANK/DQ080081
  • GENBANK/DQ091200
  • GENBANK/DQ217793
  • GENBANK/DQ269468
  • GENBANK/DQ664501
  • GENBANK/EF467176
  • GENBANK/EU240894
  • GENBANK/EU240895
  • GENBANK/EU360723
  • GENBANK/EU541441
  • GENBANK/EU541442
  • GENBANK/FJ492742
  • GENBANK/FJ804072
  • GENBANK/FM212639
  • GENBANK/GQ180114
  • GENBANK/GQ845441
  • GENBANK/GQ845443
  • GENBANK/GQ845445
  • GENBANK/GU117620
  • GENBANK/GU117621
  • GENBANK/GU117622
  • GENBANK/GU117629
  • GENBANK/GU117630
  • GENBANK/GU117631
  • GENBANK/GU117632
  • GENBANK/GU117633
  • GENBANK/GU117634
  • GENBANK/GU129016
  • GENBANK/GU225708
  • GENBANK/GU233853
  • GENBANK/GU808564
  • GENBANK/HM011570
  • GENBANK/HM999987
  • GENBANK/HM999988
  • GENBANK/HM999989
  • GENBANK/HM999990
  • GENBANK/HM999991
  • GENBANK/HM999992
  • GENBANK/HM999993
  • GENBANK/HM999994
  • GENBANK/HM999996
  • GENBANK/HM999998
  • GENBANK/HM999999
  • GENBANK/HQ625440
  • GENBANK/JF834523
  • GENBANK/JF906559
  • GENBANK/JF966371
  • GENBANK/JF966372
  • GENBANK/JF966373
  • GENBANK/JF966374
  • GENBANK/JF966375
  • GENBANK/JF966376
  • GENBANK/JF966378
  • GENBANK/JF966379
  • GENBANK/JN171845
  • GENBANK/JN211193
  • GENBANK/JN709469
  • GENBANK/JN709470
  • GENBANK/JN709471
  • GENBANK/JN709472
  • GENBANK/JX141478
  • GENBANK/JX413104
  • GENBANK/JX413105
  • GENBANK/JX413106
  • GENBANK/JX413107
  • GENBANK/JX413108
  • GENBANK/JX413109
  • GENBANK/JX413110
  • GENBANK/JX429973
  • GENBANK/JX444072
  • GENBANK/JX444073
  • GENBANK/KC108721
  • GENBANK/KC108722
  • GENBANK/KC113191
  • GENBANK/KC311731
  • GENBANK/M17463
  • GENBANK/U31789
  • GENBANK/U31790
  • GENBANK/U31792
  • GENBANK/X05817
  • GENBANK/X70827
  • GENBANK/X70829
  • GENBANK/X74464

Grant support

This work was supported by the Swedish Cancer Society (http://www.cancerfonden.se/), grant CAN2009/867, and by the Skåne Regional Research Funds (http://www.skane.se), grant ALF12711. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.