Complete genome sequence of the cyanide-degrading bacterium Pseudomonas pseudoalcaligenes CECT5344

J Biotechnol. 2014 Apr 10;175:67-8. doi: 10.1016/j.jbiotec.2014.02.004. Epub 2014 Feb 16.


Pseudomonas pseudoalcaligenes CECT5344, a Gram-negative bacterium isolated from the Guadalquir River (Córdoba, Spain), is able to utilize different cyano-derivatives. Here, the complete genome sequence of P. pseudoalcaligenes CECT5344 harboring a 4,686,340bp circular chromosome encoding 4513 genes and featuring a GC-content of 62.34% is reported. Necessarily, remaining gaps in the genome had to be closed by assembly of few long reads obtained from PacBio single molecule real-time sequencing. Here, the first complete genome sequence for the species P. pseudoalcaligenes is presented.

Keywords: Bioplastic; Cyanide; Cyanide assimilation; Cyanide resistance; Nitrilase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chromosomes, Bacterial
  • Cyanides / metabolism*
  • Genes, Bacterial
  • Genome, Bacterial*
  • Molecular Sequence Data
  • Pseudomonas pseudoalcaligenes / classification
  • Pseudomonas pseudoalcaligenes / genetics*
  • Pseudomonas pseudoalcaligenes / isolation & purification*
  • Sequence Analysis, DNA


  • Cyanides

Associated data

  • GENBANK/HG916826