Optimal temperature for synthesis of DNA, RNA, and protein by human testis in vitro

Arch Androl. 1988;20(1):41-4. doi: 10.3109/01485018808987051.


To determine the optimal temperature for DNA, RNA, and protein syntheses in the human testis, the levels of incorporation of 3H-thymidine, 14C-uridine, and 14C-leucine into testicular tissue were studied at 28 degrees C, 31 degrees C, 34 degrees C, and 37 degrees C in vitro. The results suggest that the DNA synthesis is at a maximum at 31 degrees C and shows delicate temperature sensitivity, whereas the RNA and protein syntheses are at a maximum at 34 degrees C or 37 degrees C and are temperature-dependent. It is concluded that the temperature sensitivity of DNA synthesis may be one of the primary causes of delicate thermal inhibition of human spermatogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • DNA / biosynthesis*
  • Humans
  • In Vitro Techniques
  • Leucine / metabolism
  • Male
  • Protein Biosynthesis*
  • RNA / biosynthesis*
  • Temperature
  • Testis / metabolism*
  • Thymidine / metabolism
  • Uridine / metabolism


  • RNA
  • DNA
  • Leucine
  • Thymidine
  • Uridine