Sustained in vivo depletion of splenic langerin(+) CD8α(+) dendritic cells is well-tolerated by lang-DTREGFP mice

J Immunol Methods. 2014 Apr;406:104-9. doi: 10.1016/j.jim.2014.02.005. Epub 2014 Feb 18.

Abstract

Splenic langerin(+) CD8α(+) dendritic cells (DCs) have exhibited a critical role in cross-priming CD8(+) T cell responses. To further study the roles of this DC subset, a protocol for the continuous depletion of langerin(+) CD8α(+) DCs was established using the pre-existing lang-DTREGFP mouse model. Due to the fast turnover rate of splenic CD8α(+) DCs, maintaining the depletion of langerin(+) CD8α(+) DCs required multiple diphtheria toxin (DT) treatments. We found that prolonged treatment with DT did not cause weight loss, or neutrophilia, as reported in some DT-based depletion models. Therefore, the in vivo depletion of murine langerin(+) CD8α(+) DCs can be maintained over time to analyse their function during the full course of an immune response.

Keywords: Dendritic cells; Depletion; Diphtheria toxin; Langerin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigen Presentation / immunology
  • Antigens, Surface / immunology
  • CD8 Antigens / immunology
  • CD8-Positive T-Lymphocytes / immunology*
  • Dendritic Cells / immunology*
  • Diphtheria Toxin / administration & dosage
  • Diphtheria Toxin / immunology*
  • Lectins, C-Type / immunology
  • Lymphocyte Activation / immunology
  • Lymphocyte Depletion*
  • Mannose-Binding Lectins / immunology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Spleen / cytology
  • Spleen / immunology

Substances

  • Antigens, Surface
  • CD8 Antigens
  • CD8alpha antigen
  • Cd207 protein, mouse
  • Diphtheria Toxin
  • Lectins, C-Type
  • Mannose-Binding Lectins