Epstein-Barr virus-transformed B cells bearing idiotypes of anti-DNA autoantibodies

J Clin Immunol. 1988 May;8(3):193-9. doi: 10.1007/BF00917566.


Epstein-Barr virus (EBV)-transformed B cells obtained from healthy subjects had the same idiotypes of anti-DNA autoantibodies on their surface as those obtained from patients suffering from systemic lupus erythematosus. These clones secreted anti-single-stranded or anti-double-stranded DNA antibodies. Among them, some produced anti-DNA idiotype-positive antibodies but failed to bind DNA. This was confirmed by a competitive inhibition radioimmunoassay. It was then considered whether or not the expression of anti-DNA idiotype on B-cell clones related to the anti-DNA antibody activity in vivo. The amounts of anti-DNA antibodies were not associated with the incidence of idiotype-positive B cells in the EBV-transformed cell lines from normals. The results indicate that the clones committed to the synthesis of anti-DNA idiotype-positive antibodies commonly exist at a resting state in the circulation of healthy subjects, probably through the self-tolerance regulatory system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Antinuclear / analysis*
  • B-Lymphocytes / immunology*
  • Cell Transformation, Viral*
  • DNA / immunology*
  • Epitopes
  • Herpesvirus 4, Human / immunology
  • Humans
  • Lupus Erythematosus, Systemic / immunology*


  • Antibodies, Antinuclear
  • Epitopes
  • DNA