Mantra 2.0: an online collaborative resource for drug mode of action and repurposing by network analysis

Bioinformatics. 2014 Jun 15;30(12):1787-8. doi: 10.1093/bioinformatics/btu058. Epub 2014 Feb 20.


Summary: Elucidation of molecular targets of a compound [mode of action (MoA)] and its off-targets is a crucial step in drug development. We developed an online collaborative resource (MANTRA 2.0) that supports this process by exploiting similarities between drug-induced transcriptional profiles. Drugs are organized in a network of nodes (drugs) and edges (similarities) highlighting 'communities' of drugs sharing a similar MoA. A user can upload gene expression profiles before and after drug treatment in one or multiple cell types. An automated processing pipeline transforms the gene expression profiles into a unique drug 'node' embedded in the drug-network. Visual inspection of the neighbouring drugs and communities helps in revealing its MoA and to suggest new applications of known drugs (drug repurposing). MANTRA 2.0 allows storing and sharing user-generated network nodes, thus making MANTRA 2.0 a collaborative ever-growing resource.

Availability and implementation: The web tool is freely available for academic use at

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cooperative Behavior
  • Drug Discovery / methods*
  • Drug Repositioning / methods*
  • Gene Expression Profiling*
  • Internet
  • Software*
  • Transcriptome / drug effects