Efficacy of influenza haemagglutinin and nucleoprotein as protective antigens against influenza virus infection in mice

Scand J Immunol. 1988 Jul;28(1):81-5. doi: 10.1111/j.1365-3083.1988.tb02418.x.

Abstract

Influenza nucleoprotein (NP)-specific cytotoxic T lymphocytes (CTL) stimulated by immunization of mice with VV-PR8-NP6, a recombinant vaccinia virus expressing A/PR/8/34 NP, did not protect mice against challenge with A/PR/8/34 4 days later. Neither were secondary NP-specific CTL stimulated by reimmunization able to protect mice. These results contrast with the ability of transferred, in vitro-cultured and stimulated, NP-specific CTL to protect recipient mice from challenge with A/PR/8/34. Immunization of mice with a recombinant vaccinia virus expressing A/PR/8/34 HA protected mice challenged 4 days later, either via the small amount of antibody already present, or via HA-specific CTL that would have to be more efficient than NP-specific CTL in either trafficking to the infected lung or in effector function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / biosynthesis
  • Antigens, Viral / administration & dosage*
  • Epitopes / immunology
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Hemagglutinins, Viral / immunology
  • Hemagglutinins, Viral / therapeutic use*
  • Immunization, Secondary
  • Influenza A virus / immunology*
  • Mice
  • Mice, Inbred CBA
  • Nucleocapsid Proteins
  • Nucleoproteins*
  • Orthomyxoviridae Infections / immunology
  • Orthomyxoviridae Infections / prevention & control*
  • T-Lymphocytes, Cytotoxic / immunology
  • Viral Core Proteins / immunology
  • Viral Core Proteins / therapeutic use*

Substances

  • Antibodies, Viral
  • Antigens, Viral
  • Epitopes
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Hemagglutinins, Viral
  • Nucleocapsid Proteins
  • Nucleoproteins
  • Viral Core Proteins