Ultrasound-directed, site-specific gene delivery

Methods Mol Biol. 2014:1141:67-76. doi: 10.1007/978-1-4939-0363-4_3.

Abstract

With the implementation of gene therapy looming in the near term, an effective delivery system using noninvasive, nonviral-mediated methods appears as an attractive option. This novel platform technology uses gas-filled, ultrasound-directed acoustic microspheres for both diagnostic imaging and therapy and yet may provide a key component for future success in the pursuit of single-gene replacement therapy.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Apolipoprotein A-I / genetics*
  • Apolipoprotein A-I / metabolism
  • Contrast Media
  • Disease Models, Animal
  • Gene Transfer Techniques*
  • Genetic Therapy / methods*
  • Humans
  • Hypoalphalipoproteinemias / genetics
  • Hypoalphalipoproteinemias / metabolism
  • Hypoalphalipoproteinemias / pathology
  • Hypoalphalipoproteinemias / therapy*
  • Injections, Intravenous
  • Lipoproteins, HDL / blood
  • Male
  • Microbubbles*
  • Microspheres
  • Plasmids / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Ultrasonics

Substances

  • Apolipoprotein A-I
  • Contrast Media
  • Lipoproteins, HDL