Profiling of RNA modifications by multiplexed stable isotope labelling

Chem Commun (Camb). 2014 Apr 4;50(26):3516-8. doi: 10.1039/c3cc49114e. Epub 2014 Feb 24.

Abstract

The combination of (15)N/(13)C stable isotope labelling (SIL) and LC-MS/MS revealed a total of 52 modifications in RNA from E. coli and yeast, including 10 previously undescribed modifications. Two modifications, N-ribosylnicotinamide and 2-methylthioadenosine, were newly detected in species hitherto thought not to contain these modifications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Liquid
  • Escherichia coli / genetics
  • Isotope Labeling*
  • RNA / chemistry*
  • Saccharomyces cerevisiae / genetics
  • Tandem Mass Spectrometry

Substances

  • RNA