High level of expression in the prostate of a human glandular kallikrein mRNA related to prostate-specific antigen

FEBS Lett. 1988 Aug 15;236(1):205-8. doi: 10.1016/0014-5793(88)80315-6.

Abstract

Using a synthetic oligonucleotide primer complementary to human prostate-specific antigen mRNA, we found that an additional sequence possibly similar to human glandular kallikrein-1 could be read by a primer-extension sequencing technique. We were able to confirm the identity of that additional sequence with another oligonucleotide primer complementary to a specific region of the human glandular kallikrein-1 mRNA sequence. Northern blot analysis with 2 oligonucleotide probes respectively specific for prostate-specific antigen and human glandular kallikrein-1 mRNAs showed that the length of both mRNAs was similar at 1.5 kb. The level of human glandular kallikrein-1 mRNA relative to that of prostate-specific antigen could be estimated as approx. 10-20%. This study constitutes the first evidence that the human glandular kallikrein-1 gene is expressed at a high level in a human tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Neoplasm / genetics*
  • Base Sequence
  • Densitometry
  • Gene Expression Regulation
  • Humans
  • Kallikreins / genetics*
  • Male
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Prostate / immunology*
  • Prostate / metabolism
  • Prostate-Specific Antigen
  • Prostatic Neoplasms / analysis
  • Prostatic Neoplasms / immunology
  • RNA, Messenger / biosynthesis*
  • RNA, Messenger / genetics

Substances

  • Antigens, Neoplasm
  • RNA, Messenger
  • Kallikreins
  • Prostate-Specific Antigen