A new murine model to study musculoskeletal tuberculosis (short communication)

Liesbeth M Kager; Jurgen H Runge; Aart J Nederveen; Joris J T H Roelofs; Jaap Stoker; Mario Maas; Tom van der Poll

doi:10.1016/j.tube.2014.01.002

A new murine model to study musculoskeletal tuberculosis (short communication)

Tuberculosis (Edinb). 2014 May;94(3):306-10. doi: 10.1016/j.tube.2014.01.002. Epub 2014 Feb 8.

Authors

Liesbeth M Kager¹, Jurgen H Runge², Aart J Nederveen³, Joris J T H Roelofs⁴, Jaap Stoker⁵, Mario Maas⁶, Tom van der Poll⁷

Affiliations

¹ Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center/University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands; Center for Experimental and Molecular Medicine (CEMM), Academic Medical Center/University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. Electronic address: l.m.kager@amc.uva.nl.
² Department of Radiology, Academic Medical Center/University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. Electronic address: j.h.runge@amc.uva.nl.
³ Department of Radiology, Academic Medical Center/University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. Electronic address: a.j.nederveen@amc.uva.nl.
⁴ Department of Pathology, Academic Medical Center/University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. Electronic address: j.j.roelofs@amc.uva.nl.
⁵ Department of Radiology, Academic Medical Center/University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. Electronic address: j.stoker@amc.uva.nl.
⁶ Department of Radiology, Academic Medical Center/University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. Electronic address: m.maas@amc.uva.nl.
⁷ Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center/University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands; Center for Experimental and Molecular Medicine (CEMM), Academic Medical Center/University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands; Division of Infectious Diseases, Academic Medical Center/University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. Electronic address: t.vanderpoll@amc.uva.nl.

PMID: 24572169
DOI: 10.1016/j.tube.2014.01.002

Abstract

Musculoskeletal tuberculosis (TB) is a severe extrapulmonary manifestation of chronic Mycobacterium (M.) tuberculosis infection. Considering increasing incidence, multi-drug resistance and associated treatment difficulties, more preclinical research is needed. In this study we developed a murine model for musculoskeletal TB. Mice, intranasally infected with M. tuberculosis, were sacrificed after ten months. Mycobacterial growth was detected in lung and femur homogenates. Ziehl-Neelsen staining of paraffin-embedded femurs showed acid-fast rods in the myelum and Magnetic Resonance Imaging demonstrated osteomyelitis and macronodular tuberculomas. This new murine model of musculoskeletal TB might be of value to further investigate immunologic and radiologic responses.

Keywords: Bone and joint tuberculosis; Magnetic resonance imaging; Mice; Musculoskeletal imaging; Pott's disease; Spinal tuberculosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bones of Lower Extremity / microbiology
Disease Models, Animal*
Female
Lung / microbiology
Magnetic Resonance Imaging
Mice, Inbred C57BL
Musculoskeletal Diseases / microbiology*
Mycobacterium tuberculosis
Osteomyelitis / microbiology
Tuberculosis / microbiology*
Tuberculosis, Osteoarticular / microbiology
Tuberculosis, Spinal / microbiology