Efficient delivery of lentiviral vectors into resting human CD4 T cells

Gene Ther. 2014 Apr;21(4):444-9. doi: 10.1038/gt.2014.5. Epub 2014 Feb 27.


Resting human CD4 T cells are highly resistant to transfection or infection with lentiviral vectors derived from the human immunodeficiency virus. We now describe a flexible and efficient approach involving virus-like particles containing simian immunodeficiency virus lentiviral gene product protein X and pseudotyping with CXCR4-tropic HIV Env. This method permits effective genetic manipulation of these cells while preserving their naturally quiescent state. This technology can also be extended to primary lymphoid cultures where authentic cellular composition and functional relationships are preserved.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD4-Positive T-Lymphocytes / metabolism*
  • CD4-Positive T-Lymphocytes / pathology
  • Gene Transfer Techniques*
  • Genetic Therapy*
  • Genetic Vectors
  • HIV-1 / genetics
  • Humans
  • Lentivirus / genetics
  • Receptors, CXCR4 / genetics
  • Receptors, CXCR4 / therapeutic use
  • Simian Immunodeficiency Virus
  • Viral Proteins / administration & dosage
  • Viral Proteins / genetics*
  • Viral Proteins / therapeutic use
  • env Gene Products, Human Immunodeficiency Virus / genetics
  • env Gene Products, Human Immunodeficiency Virus / therapeutic use


  • Receptors, CXCR4
  • Viral Proteins
  • env Gene Products, Human Immunodeficiency Virus