Protein structure modeling with MODELLER

Methods Mol Biol. 2014:1137:1-15. doi: 10.1007/978-1-4939-0366-5_1.

Abstract

Genome sequencing projects have resulted in a rapid increase in the number of known protein sequences. In contrast, only about one-hundredth of these sequences have been characterized at atomic resolution using experimental structure determination methods. Computational protein structure modeling techniques have the potential to bridge this sequence-structure gap. In this chapter, we present an example that illustrates the use of MODELLER to construct a comparative model for a protein with unknown structure. Automation of a similar protocol has resulted in models of useful accuracy for domains in more than half of all known protein sequences.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Internet
  • Models, Molecular*
  • Protein Conformation*
  • Proteins / chemistry*
  • Software*

Substances

  • Proteins