The large neurofilament subunit (NF-H) of the rat: cDNA cloning and in situ detection

Biochem Biophys Res Commun. 1988 Aug 15;154(3):1099-106. doi: 10.1016/0006-291x(88)90254-9.


A lambda gt11 expression library was screened with a polyclonal antiserum directed against Wla Wolfgram protein which corresponds to the 2',3'-cyclic nucleotide 3'-phosphohydrolase. This antiserum also recognizes epitopes of the high protein subunit of neurofilaments (NF-H). An NF-H cDNA clone (pNF-H1: 1.7 kb) was isolated and characterized. Using pNF-H1 as a probe, a second cDNA clone (pNF-H2: 3 kb) was isolated from the lambda gt11 library. The two clones were sequenced and pNF-H2 was found to encode 80% of rat NF-H protein (coil-2 and C-terminal region). The C-terminal region contains an unusual sequence with stretches of repeats of Lys-Ser-Pro which represent possible phosphorylation sites. Specific localization in neurons of the corresponding mRNA was demonstrated by in situ hybridization using the pNF-H1 as a probe.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain Chemistry
  • Cloning, Molecular*
  • Cytoskeleton* / analysis
  • DNA / genetics*
  • DNA Restriction Enzymes
  • Immune Sera
  • Intermediate Filaments* / analysis
  • Macromolecular Substances
  • Molecular Sequence Data
  • Myelin Proteins / immunology
  • Nucleic Acid Hybridization
  • Poly A / genetics
  • RNA / genetics
  • RNA, Messenger
  • Rats


  • Immune Sera
  • Macromolecular Substances
  • Myelin Proteins
  • RNA, Messenger
  • Wolfgram proteins
  • Poly A
  • RNA
  • DNA
  • DNA Restriction Enzymes

Associated data

  • GENBANK/M21964