Development of a two-step multiplex PCR assay for typing of capsular polysaccharide synthesis gene clusters of Streptococcus suis

J Clin Microbiol. 2014 May;52(5):1714-9. doi: 10.1128/JCM.03411-13. Epub 2014 Feb 26.


We developed a practical and easy two-step multiplex PCR assay to aid in serotyping of Streptococcus suis. The assay accurately typed almost all of the serotype reference strains and field isolates of various serotypes and also identified the genotypes of capsular polysaccharide synthesis gene clusters of some serologically nontypeable strains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Capsules / genetics*
  • Genes, Bacterial / genetics*
  • Humans
  • Multiplex Polymerase Chain Reaction / methods*
  • Polysaccharides / genetics*
  • Serotyping / methods
  • Streptococcal Infections / diagnosis*
  • Streptococcal Infections / microbiology
  • Streptococcus suis / genetics*
  • Swine / microbiology
  • Swine Diseases / diagnosis
  • Swine Diseases / microbiology


  • Polysaccharides